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Can GBA insertion be done before beating the game?

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GBA insertion is when you insert a copy of Pokemon Ruby, Sapphire, Emerald, FireRed, or LeafGreen into your DS and can make certain Pokemon appear in certain routes in Gen IV games (Platinum, Pearl or Diamond).

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You need the National Dex first.

The majority of Pokémon that can be obtained in this way are in some way version-exclusive Pokémon to one of the Generation III games. With one of the five Game Boy Advance games inserted into the GBA slot of a Nintendo DS while playing either Diamond or Pearl, players will be able to find Pokémon not otherwise available in Generation IV in certain areas of Sinnoh, but only after they have unlocked the ability to use Pal Park as well. One does not need to have the game in their GBA slot the whole time; one just needs to have it in at the main screen, at which time one can simply take the cartridge out, as long as one doesn't turn the power off before catching the Pokémon.

Pal Park can be unlocked after you get National Dex!

Source - Bulbapedia
Hope this helps!

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