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Items:
Note: NPC= non player character
Adamant Orb – Marvelous Bridge (post-game, received from Shadow Triad)
Amulet Coin – Castelia City (received from dancers after battling all three)
Choice Scarf –Castelia City (gift from NPC in Pokemon Center after trading 30 or more Pokemon))
Cleanse Tag – Icirrus City (gift from NPC)
Damp Rock – Route 8 (talk to Parasol Lady west of Tubeline Bridge during the morning – available once per day)
Destiny Knot – Opelucid City (received from NPC in house east of gym)
Draco Plate – Route 13 (requires Surf – from NPC inside house west of move tutor house)
Eviolite – Castelia City (from NPC with Patrat in northernmost building)
Exp. Share – Castelia City (received from Battle Company's president), Icirrus City (pokemon fan club)
Expert Belt – Driftveil Market (from NPC if you show a Pokemon level 30 or higher)
Float Stone – Opelucid City (received from NPC in Drayden's House)
Griseous Orb – Marvelous Bridge (post-game, received from Shadow Triad)
Heat Rock – Route 8 (talk to Parasol Lady west of Tubeline Bridge during the day – available once per day)
Icy Rock – Route 8 (talk to Parasol Lady west of Tubeline Bridge at night – available once per day)
King's Rock – Route 13 (from Treasure Hunter)
Lucky Egg – From Professor Juniper during story when you meet her in
Lucky Punch – Route 13 (Treasure Hunter)
Lustrous Orb – Marvelous Bridge (post-game, received from Shadow Triad)
Macho Brace – Nimbasa City (from NPC in eastern gate)
Magmarizer – Route 13 (Treasure Hunter)
Metal Powder – Route 13 (Treasure Hunter)
Miracle Seed –Nacrene City (from NPC in westernmost house if you answer Snivy)
Mystic Water – Nacrene City (from NPC in westernmost house if you answer Oshawott)
NeverMeltIce – Cold Storage; rarely on wild Crygonal
Protector –Route 13 (Treasure Hunter)
Quick Claw – Skyarrow Bridge gate (from NPC)
Reaper Cloth – Route 13 (Treasure Hunter)
Ring Target – Opelucid City (received from NPC in Drayden's House)
Rocky Helmet – Cold Storage(outside, NCP)
Sharp Beak – Mistralton City (NPC in Cargo Service building),
Shell Bell – Driftveil City (received from NPC in house east of Driftveil Market if you've seen 50 or more Pokemon)
Smoke Ball – Castelia City (from NPC in building across from the gym)
Smooth Rock – Route 8 (talk to Parasol Lady west of Tubeline Bridge during the evening – available once per day)
Soft Sand – Desert Resort (from NPC)
Soothe Bell – Nimbasa City (show a Pokemon with high Happiness to NPC in building north of Battle Institute)
Splash Plate – Route 13 (requires Surf – from NPC inside house west of move tutor house)
Stick – Route 13 (Treasure Hunter)
Thick Club – Route 13 (Treasure Hunter)
Wide Lens –Castelia City (gift from NPC in Pokemon Center after trading 5 or more Pokemon)
Zoom Lens -- Castelia City (gift from NPC in Pokemon Center after trading 20 or more Pokemon)

Master Ball – Gift from Professor Juniper; Castelia City (gift from NPC in Pokemon Center after trading 50 or more Pokemon)
http://www.gamesradar.com/f/pokemon-black-and-white-item-location-guide/a-20110310112141116005/p-2

Evolution Items:
Dragon Scale – Route 13 (Treasure Hunter)
Dusk Stone –Route 10 (from NPC)
Electirizer –Route 13 (Treasure Hunter)
Fire Stone – Castelia City (NPC on easternmost pier if you pick Pansear)
Leaf Stone –Route 6 (research facility if you show scientist all four Deerling forms), Castelia City (NPC on easternmost pier if you pick Pansage)
Magmarizer – Route 13 (Treasure Hunter)
Moon Stone – Pinwheel Forest (from Lenora during story)
Prism Scale –Undella Town (NPC in Pokemon Center)
Protector –Route 13 (Treasure Hunter)
Reaper Cloth – Route 13 (Treasure Hunter)
Shiny Stone – Route 6 (from NPC in healing house)
Sun Stone – Nimbasa City (NPC in building north of Pokemon Center)
Thunderstone – Shopping Mall Nine (received from NPC)
Up-Grade – Route 13 (Treasure Hunter)
Water Stone – Castelia City (NPC on easternmost pier if you pick Panpour)

Misc Medicine:
Lava Cookie – Castelia City (Royal Unova on Mondays, Wednesdays and Fridays)
Old Gateau – Dragonspiral Tower (from Cedric Juniper during story), Castelia City (Royal Unova on Thursday)
RageCandyBar – Castelia City (Royal Unova on Saturday), Prof Juniper at Desert Resort
Rare Candy –Castelia City (Royal Unova on Sunday), man in Anville town

Other:
Black Flute – Route 13 (Treasure Hunter)
Blue Flute – Route 13 (Treasure Hunter)
Red Flute – Route 13 (Treasure Hunter)
Shoal Salt – Route 13 (Treasure Hunter)
Shoal Shell – Route 13 (Treasure Hunter)
White Flute – Route 13 (Treasure Hunter)
Yellow Flute – Route 13 (Treasure Hunter)
Heart Sclae- Drifveil city (If you show a girl the move she requests)

http://www.gamesradar.com/f/pokemon-black-and-white-item-location-guide/a-20110310112141116005/p-3

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(I had to do this seperatly because of the 8000 character limit.  I'm not 100% sure that you want these, but tms kind of are items...)

TMs:
TM01 (Hone Claw)
Location: Cold Storage. Given to you by Plasma Sage Vio in the very back container, where you fought against Team Plasma during your first visit.
Available After: Post-Game

TM04 (Calm Mind)
Location: Relic Castle. Given to you by Plasma Sage Ryokushi in the catacombs right before Volcarona.
Available After: Post-Game

TM08 (Bulk Up)
Location: Route 14. Given to you by Plasma Sage Jaro atop one of the waterfalls on the route. Requires Surf and
Waterfall.
Available After: Post-Game

TM10 (Hidden Power)
Location: Nuvema Town. Given to you by Prof. Juniper after having seen 115 Pokemon on your Pokedex.
Available After: 8 Badges (usually, but varies)

TM17 (Protect)
Location: Nuvema Town. Given to you by Prof. Juniper after having seen 60 Pokemon on your Pokedex.
Available After: 5 Badges (usually, but varies)

TM31 (Brick Break)
Location: Icirrus City. Talk to the old lady in the Pokemon Center lobby.
Available After: 6 Badges

TM32 (Double Team)
Location: Route 18. Given to you by Plasma Sage Rotto on the beach in the southeast corner of Route 18. Requires Surf.
Available After: Post-Game

TM40 (Aerial Ace)
Location: Mistralton City. After earning the Jet Badge, talk to the Youngster inside of the house directly to the north of the Pokemon Center. Afterwards, go west to the airport's runway and you'll find the TM in the southwest corner.
Available After: 6 Badges

TM42 (Facade)
Location: Route 8. On the eastern portion of the route. Talk to one of the Parasol Ladies there and she'll give you the TM.
Available After: 7 Badges

TM43 (Flame Charge)
Location: Tubeline Bridge. Talk to the Battle Girl running up and down the bridge.
Available After: 7 Badges
TM44 (Rest)
Location: Castelia City. In the easternmost alley, take the elevator in the second building from the left (if you approach from the southern part) to the 11F, then talk to the Hiker up there.
Available After: 2 Badges

TM45 (Attract)
Location: Castelia City. In the northern part of Castelia City, near the gate to Route 4, go into one of the buildings on the right and up the elevator. Talk to one of the girls in the bottom-right corner to receive the TM.
Available After: 2 Badges

TM49 (Echoed Voice)
Location: Nimbasa City. Talk to one of the girls in the eastern side of the Pokemon Musical Hall.
Available After: 3 Badges

TM54 (False Swipe)
Location: Nuvema Town. Given to you by Prof. Juniper after having seen 30 Pokemon on your Pokedex.
Available After: 3 Badges (usually, but may vary)
TM56 (Fling)


Location: Route 9. Thrown to you by a baseball player as soon as you enter the route.
Available After: 7 Badges

TM57 (Charge Beam)
 Location: Route 7. Talk to a lady on the route to receive it.
Available After: 5 Badges

TM62 (Acrobatics)
Location: Mistralton Gym. Given to you by the Gym Leader after your victory.
Available After: 6 Badges

TM67 (Retaliate)
Location: Nacrene Gym. Given to you by the Gym Leader after your victory.
Available After: 2 Badges

TM69 (Rock Polish)
Location: Chargestone Cave. Given to you by Plasma Sage Sumura in the optional chasm area by several Cool Trainers, in the very southern end of the floor.
Available After: Post-Game

TM70 (Flash)
Location: Castelia City. Given to you by a man in one of the alleys. Found in the third alley from the east.
Available After: 2 Badges

TM72 (Volt Switch)
Location: Nimbasa Gym. Given to you by the Gym Leader after your victory.
Available After: 4 Badges

TM75 (Swords Dance)
Location: Dreamyard. Given to you by Plasma Sage Asura after passing through the hidden basement of the Dreamyard, found where the orange traffic cone once was before beating the Elite Four.
Available After: Post-Game

TM76 (Struggle Bug)
Location: Castelia Gym. Given to you by the Gym Leader after your victory.
Available After: 3 Badges

TM78 (Bulldoze)
Location: Chargestone Cave. Given to you by Clay outside of the Chargestone Cave after earning your 5th Gym Badge.
Available After: 5 Badges

TM79 (Frost Breath)
Location: Icirrus City. Given to you by the Gym Leader after your victory.
Available After: 7 Badges

TM82 (Dragon Tail)
Location: Opelucid Gym. Given to you by the Gym Leader after your victory.
Available After: 8 Badges

TM83 (Work Up)
Location: Striaton Gym. Given to you by the Gym Leader after your victory.
Available After: 1 Badge

TM89 (U-Turn)
Location: Route 13. Given to you by the girl with the Wingull on top of the northern cliff after gathering all 3 Grams from other Trainers on the route.
Available After: Post-Game

TM94 (Rock Smash)
Location: Pinwheel Forest (Outside). Outside of the forest, along the path, talk to the lady standing at the edge of the road and she'll give you the TM.
Available After: 1 Badge

TM95 (Snarl)
Location: Trade a lock capsule with Mr. Lock in Northern Road of Castelia City
Available After: Unavailable

HM01 (Cut)
Location: Striaton City. Given to you by Fennel after earning your 1st Gym Badge.
Available After: 1 Badge

HM02 (Fly)
Location: Driftveil City. Given to you by Bianca after fighting her once you've earned your 5th Gym Badge.
Available After: 5 Badges

HM03 (Surf)
Location: Twist Mountain. Given to you by Alder after fighting Cheren once you've earned your 6th Gym Badge.
Available After: 6 Badges

HM04 (Strength)
Location: Nimbasa City. Inside of one of the apartments on the west side of town, talk to a guy with blue hair and he'll give you the HM.
Available After: 3 Badges

HM06 (Dive)
Location: Undella Town. Talk to a girl outside on the cliff and she'll give you the HM.
Available After: Post-Game

http://pokemon.marriland.com/black_white/items/list/tms_and_hms/
Also note, this does not include items, ect that you can buy from people.
My god, what an excellent answer! +1 vote
Really, PB10?  Really?
I just cant believe how fritjof wrote this really long and good answer and at final he doenst get selected!