Meta Battle Subway PokeBase - Pokemon Q&A
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Once again, this question is asked out of curiosity :)

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>Assurance's power is 2x if the target has received damage of any sort before Assurance is used. This includes Life Orb, Spikes, Stealth Rock, etc.

So yes, Life Orb contributes to the Assurance power boost.

Hope I helped. :)
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Alright, thanks! :)