I might as well stick with my theme of asking Combee questions :)
Anyway, since only the female evolves, will the eviolite work on the male ones, since their species technically has an evolution.
I'm not exactly sure why you would want an eviolite Combee, but please just humour me.
Yes it does
I just chucked 252 EVs on SDef and Def on a male combee with eviolite in Pokemon Online, went into a battle and found out I had 274 Def and SDef... when the max is 183 normally. Yay :D