Meta Battle Subway PokeBase - Pokemon Q&A
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I tried for a few times, but they still didn't get Pokerus. There's a pink smiley face so they had Pokerus before. Can I give them again so that I can use them to spread to other Pokemon?

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No

>Once a Pokémon's immune system has fought off the virus, they cannot spread it further, nor can it be spread to them by other Pokémon.
-Bulbapedia

You cannot use it to spread Pokérus to others, but it can still get the double EVs boost.

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You beat me! xD +1
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Once cured, the Pokemon cannot get Pokérus

If Pokémon that have already been infected and cured from Pokérus are in the main party and are on either side of a Pokémon currently with the disease, Pokérus will not spread to or through them after any amount of battles.

Bulbapedia

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