PokéBase - Pokémon Q&A
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All Herbal Medicine tastes bitter has the secondary effect of lowering a Pokemon's happiness. However, what if I use one on a Pokemon with, say, a Calm nature? A Calm nature boosts Special Defense, so the food it likes is bitter. Will its happiness be affected or not?

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Yes it still will be effected, nature and taste have nothing to do with it. Happiness will be lowered.

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No.
Herbs will always lower happiness, even if your Pokemon LOVES bitter foods.

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