PokéBase - Pokémon Q&A
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In most cases, healing a Pokémon of status will nullify its Guts boost. However, there is one exception.

In Generations III and IV, if a Pokémon with Guts is frozen, and it uses a move that thaws it out, that move will receive the Guts boost. This only applies on the turn the Pokémon thaws.

Guts (Bulbapedia)

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Do you know how this mechanic interacts with multi hit moves or with instruct?
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No. It returns to normal, since the status is needed for it to work.

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