Yes.
As long as a Pokemon either has friendship less than max or EVs in the stat the berry corresponds to, you can use it on that Pokemon.
When a Pokemon with max friendship is given an EV-reducing berry, the message is "[Pokemon] adores you! It lost base points for [stat]."
When a Pokemon has both max friendship and zero EVs in the corresponding stat, the Berry cannot be used, with the message "It won't have any effect."