PokéBase - Pokémon Q&A
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Does it matter what level I pump some vitamins into my Pokemon, I'm assuming you would do this at the lowest level possible?

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Which game are you playing?
Ultimately, when your Pokemon reaches level 100, it won't matter when they consumed vitamins (or when they gained EVs in general).
Last time I want to give a Protein to my Serperiro but it syas that it will have no effect.
That's because your Serperior already had 100 physical attack EVs and can't gain more from vitamins.

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You want to try and do this at the lowest possible level, yes. But you see a better effect (Less EVs per stat to raise the stat) at higher levels.

So just do it as soon as you can, as it won’t have any negative impact.

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