PokéBase - Pokémon Q&A
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Does it matter what level I pump some vitamins into my Pokemon, I'm assuming you would do this at the lowest level possible?

Which game are you playing?
Ultimately, when your Pokemon reaches level 100, it won't matter when they consumed vitamins (or when they gained EVs in general).
Last time I want to give a Protein to my Serperiro but it syas that it will have no effect.
That's because your Serperior already had 100 physical attack EVs and can't gain more from vitamins.

1 Answer

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You want to try and do this at the lowest possible level, yes. But you see a better effect (Less EVs per stat to raise the stat) at higher levels.

So just do it as soon as you can, as it won’t have any negative impact.