PokéBase - Pokémon Q&A
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I would guess no, because the anime and video game are in different branches of the pokemon tree. So, team rocket-as in all of it, is part of the kanto journey, where as Jessie, James, and Meowth-tv characters, would only appear in the tv series.

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You actually can face off against Jessie and James in the games, in pokemon yellow. However, they refer to them as "rocket" without actually revealing their name. But the fact that they use remarkably similar sprites, and that they have an Ekans, Meowth, and Koffing is a strong reference to the series.


Note how they say "surrender now or prepare to fight" before battling, and Team rocket blast off at the speed of light" at the end.