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Are there any of these that can increase the chances of Guillotine hitting?
- Moves
- Items
- Abilities
If there are, please let me know if any of them stack.

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I know that OHKO moves are unaffected by stat changes to evasion/accuracy, although OHKO moves can still be affected by No Guard, Lock-On, and Mind Reader, so I'm not sure if items or abilities can affect it.
As far as I know, you could give said Pokemon the scope lens (Maybe the other lens, I get them mixed up). also, Telekinesis on the opponent would help. It decreases their evasion but I'm fairly certain it affect OHKO moves. And compound eyes. Sorry, I'm thinking these as I type.
If you're not sure, why are you answering?
Wide Lens gives a 10% accuracy boost to moves. I use it for my Tsareena, her High Jump Kick's accuracy is usually 90 so it becomes 99 and Power Whip's is 85 so it becomes 93.5. They're high powered moves and it makes a big difference being able to use them reliably.
Are you sure wide lens is the only way? The Delphox person wanted at least 85% accuracy, and wide lens raises guillotine's accuracy to only 33%.
I remember in gen 5 I used an Excadrill with Hone Claws, Fissure and Horn Drill and after I used Hone Claws 6 times I landed the OHKO move every time. Idk if this was changed in later generations
@sumwun Thank you, you are correct. For some reason I didn't realise its accuracy was so low, I should've checked before posting, my apologies. In that case, I'd say the only viable option is X Accuracy as it would increase it by one stage (50%) up to 6 times. However, I've looked in to what @KRLW890 commented and it seems that items haven't worked for OHKO moves since Gen 3.
So this answer is wrong? Do you want to convert it to a comment?
Opponents that would let you set up 6 Hone Claws are probably vastly underleveled compared to you, and those moves come with an accuracy bonus of 1% per level of advantage you have, which probably contributed something to the results. The accuracy boost from Hone Claws, on the other hand, did not.
Because there's a high likelihood that at least one of them is accurate.
an that helps somewhat
Sure but would like to hear your answer. Edit: It converted to the wrong spot...
@shaggy Answering a question takes it off the unanswered list, which means other people who know the answer are less likely to see this question. So even though it helps somewhat, I think putting it in an answer hurts the question more than it helps.
I just edited my question to be more specific. Before answering, please note that OHKO moves are normally unaffected by changes to accuracy and evasion stats, but are affected by moves like Lock-On.

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The TLDR: Base accuracy of the guillotine OHKO is 30%, only affected by moves in after gen I & II. Difference in level between user and target affects accuracy from gen II and beyond.

More in-depth explanation:

Gen I: OHKOs are affected by accuracy/evasion stats, but auto fail if user's Speed stat is lower than targets (X Accuracy guarantees a hit)

Gen II: OHKOs auto fail is target is higher level than user's. The accuracy increases a small amount for each level the user is above the target. (X Accuracy guarantees a hit)

Gen III & Beyond: OHKOs auto fails when user is lower leveled than target. Accuracy and evasion stats do not affect OHKOs but the accuracy still goes up by a small amount for each level the user is above the target.

OHKO accuracy is not affected by: Wide Lens or abilities (With the exception of "No Guard")

OHKO are affected by moves like "Lock-On" and "Mind Reader"

This info is from Bulbapedia. A great resource to check before posting or waiting for answers to questions. :D

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Isn't it affected by no guard?
Yeah!  Well that's embarrassing :P.  Misinterpreted it as a move the way it was worded on bulbapedia!  Good Catch sumwum :D  Answer edited.